Chromatography Column, with PTFE Stopcock and Socket

Chromatography Column, with PTFE Stopcock and Socket

  • Glass chromatography columns for laboratory separations
  • Diameter range: 10 mm to 60 mm
  • Length range: 150 mm to 900 mm
  • Socket sizes: B14/23 to B40/38 for secure fittings
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Description

A chromatography column with PTFE stopcock and socket is a laboratory glassware used in gravity-based or low-pressure column chromatography. This design is optimized for secure, leak-free connections to other apparatus, such as a reservoir, fraction collector, or vacuum line, enhancing functionality in both preparative and analytical chromatography.

1. Key Components and Their Functions

Component Description
Glass Column Body A cylindrical borosilicate tube that holds the stationary phase and sample.
PTFE Stopcock A precision valve made of polytetrafluoroethylene (PTFE), chemically inert and used to regulate solvent flow.
Socket (Ground Glass Joint) A standardized ground glass joint (e.g., 24/29 or 29/32) at the top of the column that allows airtight connection to other glassware, such as solvent reservoirs, funnels, or adapters.

2. Working Principle

This chromatography setup is based on differential adsorption or partitioning between two phases:

  • Stationary Phase: A solid adsorbent (e.g., silica gel or alumina) packed into the column.

  • Mobile Phase: A solvent or solvent mixture that flows through the column, carrying the sample mixture.

Steps:

  1. Packing: The stationary phase is added as a slurry or dry-packed into the column.

  2. Connection: The socket is used to connect a reservoir or funnel containing the mobile phase.

  3. Sample Loading: The sample is applied to the top of the stationary phase.

  4. Elution: The mobile phase is introduced and controlled via the PTFE stopcock.

  5. Fraction Collection: Separated components are eluted and collected based on their migration rates.

3. Function of the Socket

Feature Benefit
Glass Socket (Ground Joint) – Enables leak-proof connection to solvent reservoirs or vacuum lines
– Allows pressurized elution or continuous solvent feed
– Enhances modularity with glass adapters, funnels, or drying tubes
– Prevents solvent evaporation or contamination during separation

4. Advantages of this Design

Component Function
PTFE Stopcock – Chemically inert
– Allows precise control of solvent flow
– Prevents leaks and degradation from solvent exposure
Glass Socket – Ensures secure, standardized connections
– Can be fitted with stoppers, septa, or adapters for closed systems

5. Applications

 Organic Synthesis & Purification

  • Isolation of desired products and removal of side products or unreacted starting materials.

Phytochemistry & Natural Product Research

  • Fractionation of plant or microbial extracts.

Environmental Chemistry

  • Extraction and separation of pollutants or biomarkers from environmental samples.

Pharmaceutical Quality Control

  • Purity profiling and separation of drug components.

Preparative Chromatography

  • Gram-scale purifications in academic and industrial laboratories.

Disclaimer: Product images are for illustration purposes only. The actual brand received may vary based on availability, as Labogemdi sources high-quality laboratory products from multiple trusted suppliers. While we are not directly affiliated with specific brands, we ensure that all products comply with laboratory and research-grade standards, meeting the requirements of academic institutions, research laboratories, and industrial applications.

 

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