Description
Thin Layer Chromatography (TLC) plates coated with Silica Gel 60, a highly porous, polar adsorbent with an average pore diameter of ~60 Å, are used as the stationary phase for separating and identifying compounds in a mixture. The silica gel is evenly coated onto a rigid glass backing for mechanical stability and precision.
- Silica Gel 60: Refers to silica with a pore size of approximately 60 Å (optimal for small to medium molecules)
- No fluorescent indicator: These plates do not contain F₂₅₄; visualization requires staining or alternative detection methods
Key Properties
| Parameter | Specification |
|---|---|
| Stationary Phase | Silica Gel 60 (SiO₂·xH₂O), binder-free |
| Pore Size | ~60 Å |
| Particle Size | ~10–15 µm |
| Layer Thickness | ~250 µm (standard) |
| Support Material | Rigid glass backing |
| Fluorescent Indicator | Not included |
| Surface Properties | Highly adsorptive, homogeneous coating |
Applications
1. Organic and Inorganic Compound Separation
- Analysis of reaction mixtures, purification intermediates
- Separation based on polarity and affinity for the silica phase
2. Natural Products and Herbal Medicine
- Monitoring plant extract compositions
- Identification of bioactive compounds without UV-active indicators
3. Educational and Research Use
- Ideal for teaching chromatography techniques
- Versatile for labs requiring chemical staining for visualization
4. Environmental and Industrial Analysis
-
Identification of organic contaminants, oil residues, or by-products
Detection Methods
Because Silica Gel 60 plates lack the F₂₅₄ fluorescent indicator, visualization of separated compounds is done via:
- Chemical staining (e.g., iodine vapor, KMnO₄, ninhydrin, vanillin)
- UV absorbance only if analytes are inherently UV-active
- Derivatization reagents for specific functional groups
Disclaimer: Product images are for illustration purposes only. The actual brand received may vary based on availability, as Labogemdi sources high-quality laboratory products from multiple trusted suppliers. While we are not directly affiliated with specific brands, we ensure that all products comply with laboratory and research-grade standards, meeting the requirements of academic institutions, research laboratories, and industrial applications.
