Description
Thin Layer Chromatography (TLC) sheets coated with Silica Gel 60 F₂₅₄ on a flexible aluminum backing. The stationary phase consists of silica gel with a particle size of 5–40 µm and a pore size of ~60 Å, impregnated with a fluorescent indicator (F₂₅₄) that fluoresces under UV light at 254 nm.
- “Silica Gel 60”: Refers to silica with 60 Å pore size
- “F₂₅₄”: Indicates incorporation of a shortwave UV-active fluorescent dye (typically zinc silicate or manganese-doped zinc silicate)
Key Properties
| Property | Details |
|---|---|
| Stationary Phase | Silica Gel 60 with F₂₅₄ fluorescent indicator |
| Backing Material | Flexible aluminum foil |
| Particle Size | 5–40 µm |
| Pore Size | ~60 Å (optimal for low- to mid-molecular-weight compounds) |
| Fluorescence Detection | Compounds quenching fluorescence appear as dark spots under 254 nm UV light |
Applications
1. Qualitative and Semi-Quantitative Analysis
- Identification of unknown compounds by comparing Rf values
- Separation and characterization of small molecules in complex mixtures
2. Visualization of UV-Active Compounds
- Detection of substances absorbing at 254 nm (e.g., aromatics, conjugated systems)
- Enhanced contrast for non-colored analytes
3. Quality Control and Purity Testing
- Pharmaceuticals, dyes, food additives, and fine chemicals
- Fast screening of multiple samples on one plate
4. Environmental and Forensic Analysis
- Separation of pesticides, PAHs, illicit substances, and degradation products
Disclaimer: Product images are for illustration purposes only. The actual brand received may vary based on availability, as Labogemdi sources high-quality laboratory products from multiple trusted suppliers. While we are not directly affiliated with specific brands, we ensure that all products comply with laboratory and research-grade standards, meeting the requirements of academic institutions, research laboratories, and industrial applications.
