Description
HPTLC (High-Performance Thin Layer Chromatography) plates coated with Silica Gel 60 F₂₅₄ are high-resolution chromatographic media designed for enhanced separation and reproducibility. The stationary phase consists of silica gel with ~60 Å pore size, optimized particle size distribution (~5–7 µm), and fluorescent indicator F₂₅₄. These are coated onto a glass backing for maximum flatness, dimensional stability, and compatibility with densitometric scanning.
Key Properties
| Parameter | Specification |
|---|---|
| Stationary Phase | Silica Gel 60 (SiO₂·xH₂O), pore size ~60 Å |
| Particle Size | ~5–7 µm (smaller than conventional TLC for better resolution) |
| Fluorescent Indicator | F₂₅₄ (fluoresces under 254 nm UV light) |
| Support Material | Glass plates (robust, flat, reusable in automated HPTLC systems) |
| Layer Thickness | Typically ~200 µm |
| Surface Properties | Smooth, highly uniform, and adherent coating |
| UV Detection | Dark spots appear on bright green background under 254 nm UV light |
Applications
1. High-Resolution Separation
- Complex sample matrices: plant extracts, synthetic mixtures, formulations
- Improved separation of closely related compounds compared to conventional TLC
2. Quantitative and Qualitative Analysis
- Used with densitometry or image analysis systems for quantification
- Rf value comparison for compound identification
3. Pharmaceuticals and Botanical Products
- Quality control of active pharmaceutical ingredients (APIs) and herbal medicines
- Standardized fingerprinting of natural products
4. Forensic, Food, and Environmental Testing
-
Pesticide residue analysis, mycotoxins, food adulterants, and trace pollutants
Disclaimer: Product images are for illustration purposes only. The actual brand received may vary based on availability, as Labogemdi sources high-quality laboratory products from multiple trusted suppliers. While we are not directly affiliated with specific brands, we ensure that all products comply with laboratory and research-grade standards, meeting the requirements of academic institutions, research laboratories, and industrial applications.
